Journal: MethodsX

Article Title: Development and evaluation of mouse anti-Ara h 1 and Ara h 3 IgE monoclonal antibodies for advancing peanut allergy research

doi: 10.1016/j.mex.2023.102470

Figure Lengend Snippet: Dose dependency of each IgE monoclonal antibody against CPE in a sandwich ELISA.

Article Snippet: All plates were screened for anti-CPE IgE antibody-producing hybridomas by ELISA kits (Cat# 3063, Chondrex, Inc. WA, USA), according to the manufacturer's instructions.

Techniques: Sandwich ELISA

Journal: MethodsX

Article Title: Development and evaluation of mouse anti-Ara h 1 and Ara h 3 IgE monoclonal antibodies for advancing peanut allergy research

doi: 10.1016/j.mex.2023.102470

Figure Lengend Snippet: Dose dependency of each IgE monoclonal antibody against CPE in an indirect ELISA.

Article Snippet: All plates were screened for anti-CPE IgE antibody-producing hybridomas by ELISA kits (Cat# 3063, Chondrex, Inc. WA, USA), according to the manufacturer's instructions.

Techniques: Indirect ELISA

Journal: MethodsX

Article Title: Development and evaluation of mouse anti-Ara h 1 and Ara h 3 IgE monoclonal antibodies for advancing peanut allergy research

doi: 10.1016/j.mex.2023.102470

Figure Lengend Snippet: Summary of the Application of Anti-CPE IgE Monoclonal Antibodies.

Article Snippet: All plates were screened for anti-CPE IgE antibody-producing hybridomas by ELISA kits (Cat# 3063, Chondrex, Inc. WA, USA), according to the manufacturer's instructions.

Techniques: Indirect ELISA, Sandwich ELISA

Journal: Mediators of Inflammation

Article Title: Cooling and Sterile Inflammation in an Oxygen-Glucose-Deprivation/Reperfusion Injury Model in BV-2 Microglia

doi: 10.1155/2021/8906561

Figure Lengend Snippet: Western blot analysis analyzing extracellular proteins and DAMPs. (a) HMGB1, (b) Hsp70, (c) CIRBP, and (d) RBM3 presented as x -fold change relative to Normoxia 37°C control and (e) representative immunoblots at respective timepoints. Cells were exposed to 2 h and 6 h of oxygen-glucose deprivation (OGD, 0.2% O 2 in glucose/serum-free medium) and incubated at 37°C or cooled 1 h after experimental start to 33.5°C. Data from at least 3 individual experiments are presented as mean ± SD. Statistical analysis was conducted using one-way analysis of variance (ANOVA) with Tukey posttest; ∗ p < 0.05 for group comparison and # p < 0.05 for comparison to Normoxia 37°C were considered significant. Panels show representative Immunoblots.

Article Snippet: Primary antibodies for β -Actin (1 : 20000, Cell Signaling, Cat#4967), Caspase 1 (1 : 1000, Cell Signaling, Cat#67314), Caspase 8 (1 : 1000, Cell Signaling, Cat#9429), Caspase 9 (1 : 1000, Cell Signaling, Cat#9508), Caspase 3 (1 : 1000, Cell Signaling, Cat#9662), Hsp70 (1 : 1000, Cell Signaling Technology, Cat#4872) HMGB1 (1 : 2000, Chondrex, Cat#7028), CIRBP (1 : 1000, Abclonal, Cat#A6080), and RBM3 (1 : 1000, Proteintech, Cat#14363-1-AP) were diluted in blocking solution and incubated overnight at 4°C.

Techniques: Western Blot, Incubation